Antimicrobial Potential of Calotropis gigantea: Synergistic Interactions with Conventional Antibiotics Against Multidrug-Resistant Bacteria

This research proposal explores the antibacterial properties of <strong>Calotropis gigantea</strong> against multidrug-resistant bacterial strains, with a focus on its potential synergistic effects when combined with conventional antibiotics. The study will employ soxhlet extraction to isolate bioactive compounds from various plant parts, followed by bioassay-guided fractionation to determine their minimum inhibitory concentrations against pathogens like MRSA. Additionally, checkerboard assays will assess potential synergistic interactions, aiming to enhance antibiotic efficacy and reduce resistance development. Cytotoxicity testing will ensure the safety of the extracts for potential topical use. The findings could offer new insights into integrating traditional medicinal plants with modern therapeutic strategies, presenting <strong>Calotropis gigantea</strong> as a viable option for combating antibiotic-resistant infections.

Objectives

The general objective of this research is to investigate and expand the understanding of the antimicrobial properties of Calotropis gigantea, specifically its efficacy against multidrug-

resistant bacterial strains and its potential to enhance the effectiveness of conventional antibiotics through synergistic interactions.

Specific Objectives:

1. Evaluate the Antibacterial Efficacy Against Multidrug-Resistant Strains:

2. To assess the effectiveness of Calotropis gigantea extracts against various multidrug-resistant bacterial strains, such as MRSA (Methicillin-resistant Staphylococcus aureus) and MDR-TB (Multidrug-resistant Mycobacterium tuberculosis), which are significant public health concerns due to their resistance to standard antibiotic treatments.

3. Investigate Synergistic Effects with Conventional Antibiotics:

4. To explore the potential synergistic effects of Calotropis gigantea extracts when combined with commonly used antibiotics. This involves determining whether these combinations can enhance the efficacy of the antibiotics, potentially lowering required doses and reducing side effects, thereby addressing issues related to antibiotic resistance.

5. Elucidate Mechanisms of Antimicrobial Action:

6. To identify the active biochemical compounds in Calotropis gigantea responsible for its antimicrobial properties and to elucidate the underlying mechanisms by which these compounds exert their effects. This includes studying interactions at the molecular level within bacterial cells, which could provide insights into how these natural products might be optimized and used more effectively in clinical settings.

7. Assess the Safety Profile of Calotropis Gigantea Extracts:

To evaluate the cytotoxicity and potential adverse effects of Calotropis gigantea extracts on human cells, ensuring that any therapeutic use of the plant’s extracts will be safe for clinical applications. This objective is crucial for translating laboratory findings into practical medical treatments.

Funding Agency:

MOHERI