Alzheimer’s disease (AD) is an irreversible progressive neurodegenerative disease affecting millions of lives worldwide. Its prevalence is on rise worldwide especially in developed countries leading to significant health, social, and financial burden on the society and the government. The project aim to identify the pomegranate peel as a source of dietary antioxidant vis a vis to evaluate its role in protection against the progression of Alzheimers Disease. An attempt is made to quantify the major flavonoid by using HPLC-RP technique.
Major Objectives: (i) To collect the peel of random, and fresh Pomegranates from Jabal Akhdar and prepare the PPE by using three organic solvents (Ethanol, Acetone and Chloroform) (ii) To perform the preliminaryphytochemical screening of PPE (iii) To estimate the total phenols and flavonoids in PPE by colorimetric methods. (iv) To investigate the antioxidant potential of PPE using suitable in vitro assay methods such as DPPH, Total Antioxidant Capacity (v) To evaluate the Acetyl cholinesterase (AchE) inhibitory activity of PPE at different concentrations (vi) To perform the HPLC-RP analysis for the most potent extract for Quercetin quantification (vii) To study the relationship between total phenolic content and antioxidant activity to identify the most effective solvent for extracting the potent antioxidant, phenolics and AchE inhibitor from pomegranate peel.
Research Team:
(i) Rehab Khokar (ii) Aliya Almamari (iii) Mariya Hasan (iv) Al Ghaliya Alfarsi (v) Dr Shah Alam Khan (Faculty Mentor)